Insertion of 39 nt at input position 215: tagattagaagaagaattaagaagaagattaacagaaag

--- primers ---
forward anneal: caagggcgaggagctg len 16 Tm 61.24
reverse anneal: ctcatatgtatatctccttcttaaagttaaac len 32 Tm 59.84
dTm = 1.40
forward primer: caagggcgaggagctg ( 16 nt )
reverse primer: ctttctgttaatcttcttcttaattcttcttctaatctactcatatgtatatctccttcttaaagttaaac ( 71 nt )
fwd: longest annealed 3' suffix = 16 nt  Tm=61.24  caagggcgaggagctg
rev: longest annealed 3' suffix = 32 nt  Tm=59.84  ctcatatgtatatctccttcttaaagttaaac
fwd anneal: caagggcgaggagctg occurs 1x in input (at 215)
rev anneal(top-strand): gtttaactttaagaaggagatatacatatgag occurs 1x in input (at 183)

product length: 3630 output length: 3630
product is rotation of output: true

wrote /app/primers.fasta:
>primer1_forward
caagggcgaggagctg
>primer1_reverse
ctttctgttaatcttcttcttaattcttcttctaatctactcatatgtatatctccttcttaaagttaaac

