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The following additional packages will be installed:
  ca-certificates krb5-locales libbrotli1 libcurl4t64 libgssapi-krb5-2
  libk5crypto3 libkeyutils1 libkrb5-3 libkrb5support0 libldap-common libldap2
  libnghttp2-14 libpsl5t64 librtmp1 libsasl2-2 libsasl2-modules
  libsasl2-modules-db libssh-4 libssl3t64 openssl publicsuffix
Suggested packages:
  krb5-doc krb5-user libsasl2-modules-gssapi-mit
  | libsasl2-modules-gssapi-heimdal libsasl2-modules-ldap libsasl2-modules-otp
  libsasl2-modules-sql ncbi-epcr
The following NEW packages will be installed:
  ca-certificates curl krb5-locales libbrotli1 libcurl4t64 libgssapi-krb5-2
  libk5crypto3 libkeyutils1 libkrb5-3 libkrb5support0 libldap-common libldap2
  libnghttp2-14 libpsl5t64 librtmp1 libsasl2-2 libsasl2-modules
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everything's installed!

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    source $HOME/.local/bin/env (sh, bash, zsh)
    source $HOME/.local/bin/env.fish (fish)
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============================= test session starts ==============================
platform linux -- Python 3.13.9, pytest-8.4.1, pluggy-1.6.0
rootdir: /tests
plugins: json-ctrf-0.3.5
collected 1 item

../tests/test_outputs.py F                                               [100%]

=================================== FAILURES ===================================
_________________________________ test_primers _________________________________

    def test_primers():
        """Test that the generated primers will successfully amplify the input DNA and
        contain the required overhangs that will result in the output sequence.
        """
    
        # Reference the directory the agent operated in (the WORKDIR in the Docker env)
        primers_path = Path("/app/primers.fasta")
        assert primers_path.exists(), f"File {primers_path} does not exist."
    
        with open(primers_path, "r") as f:
            lines = [line.rstrip() for line in f]
    
        # This task only needs one primer pair to solve each primer takes up
        # two lines so the fasta file should only have 4 lines in total.
        assert len(lines) == 4, "Invalid number of lines in primers.fasta."
        fwd_primer = lines[1].lower()
        rev_primer = lines[3].lower()
        # If any other character is included in the sequence then this isn't a
        # valid synthesizable primer.
        assert re.fullmatch(r"[atcg]+", fwd_primer), "Primer must contain only A, T, C, G."
        assert re.fullmatch(r"[atcg]+", rev_primer), "Primer must contain only A, T, C, G."
    
        # Concatenatenating these two primers should give us the following
        # sequence: input left overlap + insert + input right overlap
        primers_concat = rc(rev_primer) + fwd_primer
        # Check that we're actually encoding the insert in the two primers.
        insert_start = primers_concat.find(insert)
        assert insert_start != -1, "Primer must contain inserted DNA."
        insert_end = insert_start + len(insert)
        annealed_rev = primers_concat[:insert_start]
        annealed_fwd = primers_concat[insert_end:]
    
        # Check the length requirement is satisfied.
        assert 15 <= len(annealed_fwd) <= 45, (
            "Annealed part of forward primer must be between 15 and 45 nucleotides."
        )
        assert 15 <= len(annealed_rev) <= 45, (
            "Annealed part of reverse must be between 15 and 45 nucleotides."
        )
    
        # The rest of primers_concat should just be the parts that can anneal
        # to the input.
        assert vector1[-len(annealed_rev) :] == annealed_rev, (
            "Reverse primer must overlap vector."
        )
        assert vector2[: len(annealed_fwd)] == annealed_fwd, (
            "Forward primer must overlap vector."
        )
    
        # Make sure that the two primers satisfy the melting temperature
        # requirements described in task.yaml.
        fwd_tm = calc_tm_oligotm(annealed_fwd)
        rev_tm = calc_tm_oligotm(rc(annealed_rev))
        assert 58 <= fwd_tm <= 72, (
            "Forward primer must have a Tm between 58 and 72 degrees C."
        )
        assert 58 <= rev_tm <= 72, (
            "Reverse primer must have a Tm between 58 and 72 degrees C."
        )
>       assert abs(fwd_tm - rev_tm) <= 5, (
            "Tm of forward and reverse primers must be within 5 degrees C of each other."
        )
E       AssertionError: Tm of forward and reverse primers must be within 5 degrees C of each other.
E       assert 8.191611000000009 <= 5
E        +  where 8.191611000000009 = abs((66.274364 - 58.082753))

/tests/test_outputs.py:100: AssertionError
=========================== short test summary info ============================
FAILED ../tests/test_outputs.py::test_primers - AssertionError: Tm of forward...
============================== 1 failed in 0.04s ===============================
